Characterization of a Temperate Streptococcus therrnophilus Bacteriophage and Its Genetic Relationship with Lyric Phages
Identifieur interne : 004206 ( Main/Exploration ); précédent : 004205; suivant : 004207Characterization of a Temperate Streptococcus therrnophilus Bacteriophage and Its Genetic Relationship with Lyric Phages
Auteurs : Harald Brüssow [Suisse] ; Anne Bruttin [Suisse]Source :
- Virology [ 0042-6822 ] ; 1995.
Abstract
Abstract: The temperate Streptococcus thermophilus bacteriophage φSFi21 showed an 38-kb-long double-stranded DNA genome with cohesive ends. A single integration site was used in lysogens established in three different S. thermophilus strains, The attP and attB sites were localized on the restriction map of phage DNA and by hybridization on pulsed field separated bacterial DNA All laboratory-established lysogens showed in addition to integrated prophage DNA unintegrated monomer phage DNA with unligated cos sites The genetic relatedness of φSFi21 DNA with DNA from lytic phages was studied in dot blot and Southern blot hybridization by using individual restriction fragments of φSFi21 DNA as probes Lyric group I phages hybridized with fragments of the central and the right part of the φSFi21 genome but failed to hybridize with a fragment joining both parts. Lyfic group II phages showed hybridization with the right half of the φSFi21 genome. In lytic group IV phages, biologically a heterogeneous group, many different combinations of cross hybridization were detected in accordance with the hypothesis of the modular evolution of phage genomes.
Url:
DOI: 10.1006/viro.1995.1521
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001793
- to stream Istex, to step Curation: 001793
- to stream Istex, to step Checkpoint: 001897
- to stream Main, to step Merge: 004267
- to stream Main, to step Curation: 004206
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of a Temperate Streptococcus therrnophilus Bacteriophage and Its Genetic Relationship with Lyric Phages</title><author><name sortKey="Brussow, Harald" sort="Brussow, Harald" uniqKey="Brussow H" first="Harald" last="Brüssow">Harald Brüssow</name></author><author><name sortKey="Bruttin, Anne" sort="Bruttin, Anne" uniqKey="Bruttin A" first="Anne" last="Bruttin">Anne Bruttin</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:C85A43EB22C531A8A6E9B1C4E553954A7309E725</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1006/viro.1995.1521</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-259H254H-S/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001793</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001793</idno><idno type="wicri:Area/Istex/Curation">001793</idno><idno type="wicri:Area/Istex/Checkpoint">001897</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001897</idno><idno type="wicri:doubleKey">0042-6822:1995:Brussow H:characterization:of:a</idno><idno type="wicri:Area/Main/Merge">004267</idno><idno type="wicri:Area/Main/Curation">004206</idno><idno type="wicri:Area/Main/Exploration">004206</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Characterization of a Temperate Streptococcus therrnophilus Bacteriophage and Its Genetic Relationship with Lyric Phages</title><author><name sortKey="Brussow, Harald" sort="Brussow, Harald" uniqKey="Brussow H" first="Harald" last="Brüssow">Harald Brüssow</name><affiliation wicri:level="1"><country xml:lang="fr">Suisse</country><wicri:regionArea>Nestlé Research Centre, Nestec Ltd., Vers-chez-les-Blanc, CH-1000 Lausanne 26</wicri:regionArea><wicri:noRegion>CH-1000 Lausanne 26</wicri:noRegion></affiliation></author><author><name sortKey="Bruttin, Anne" sort="Bruttin, Anne" uniqKey="Bruttin A" first="Anne" last="Bruttin">Anne Bruttin</name><affiliation wicri:level="1"><country xml:lang="fr">Suisse</country><wicri:regionArea>Nestlé Research Centre, Nestec Ltd., Vers-chez-les-Blanc, CH-1000 Lausanne 26</wicri:regionArea><wicri:noRegion>CH-1000 Lausanne 26</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">212</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="632">632</biblScope><biblScope unit="page" to="640">640</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The temperate Streptococcus thermophilus bacteriophage φSFi21 showed an 38-kb-long double-stranded DNA genome with cohesive ends. A single integration site was used in lysogens established in three different S. thermophilus strains, The attP and attB sites were localized on the restriction map of phage DNA and by hybridization on pulsed field separated bacterial DNA All laboratory-established lysogens showed in addition to integrated prophage DNA unintegrated monomer phage DNA with unligated cos sites The genetic relatedness of φSFi21 DNA with DNA from lytic phages was studied in dot blot and Southern blot hybridization by using individual restriction fragments of φSFi21 DNA as probes Lyric group I phages hybridized with fragments of the central and the right part of the φSFi21 genome but failed to hybridize with a fragment joining both parts. Lyfic group II phages showed hybridization with the right half of the φSFi21 genome. In lytic group IV phages, biologically a heterogeneous group, many different combinations of cross hybridization were detected in accordance with the hypothesis of the modular evolution of phage genomes.</div></front></TEI><affiliations><list><country><li>Suisse</li></country></list><tree><country name="Suisse"><noRegion><name sortKey="Brussow, Harald" sort="Brussow, Harald" uniqKey="Brussow H" first="Harald" last="Brüssow">Harald Brüssow</name></noRegion><name sortKey="Bruttin, Anne" sort="Bruttin, Anne" uniqKey="Bruttin A" first="Anne" last="Bruttin">Anne Bruttin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004206 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004206 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:C85A43EB22C531A8A6E9B1C4E553954A7309E725
|texte= Characterization of a Temperate Streptococcus therrnophilus Bacteriophage and Its Genetic Relationship with Lyric Phages
}}
| This area was generated with Dilib version V0.6.33. |  |